Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 10051570.
- Also identified by PMC identifier 26712.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After treatment of cells with DNA-damaging agents or gamma irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 60 min. The mammalian recombination protein Rad51 and the replication protein A then accumulate at sites of ssDNA and form foci, suggesting that these are sites of recombinational DNA repair.
Medical subject headings
- Cell Cycle
- Cell Nucleus
- DNA Damage
- DNA, Single-Stranded
- Nuclear Proteins