A recombinant, fully human monoclonal antibody with antitumor activity constructed from phage-displayed antibody fragments.
basic_science · Level V
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Abstract
A single-chain Fv antibody fragment specific for the tumor-associated Ep-CAM molecule was isolated from a semisynthetic phage display library and converted into an intact, fully human IgG1 monoclonal antibody (huMab). The purified huMab had an affinity of 5 nM and effectively mediated tumor cell killing in in vitro and in vivo assays. These experiments show that nonimmunized phage antibody display libraries can be used to obtain high-affinity, functional, and clinically applicable huMabs directed against a tumor-associated antigen.
Medical subject headings
- Antibodies, Monoclonal
- Antigens, Neoplasm
- Antineoplastic Agents
- Cell Adhesion Molecules
- Colonic Neoplasms
- Immunoglobulin Fragments
- Molecular Biology