Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
basic_science · Level V
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Abstract
The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1-depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.
Medical subject headings
- Cell Cycle Proteins
- GTP Phosphohydrolases
- Guanine Nucleotide Exchange Factors
- Guanosine Triphosphate
- Microtubules
- Nuclear Proteins
- Spindle Apparatus
- Xenopus Proteins