Technique for harvesting keratolimbal allografts from corneoscleral buttons.
basic_science · Level V
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- Record sourced from PubMed, PMID 10458185.
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Abstract
To report a method for obtaining thin keratolimbal lenticules from cadaveric corneoscleral buttons for corneal epithelial stem cell allografts. A standard silicone orbital sizing sphere and three 25-gauge needles were employed to fix the corneoscleral button to facilitate excision of keratolimbal lenticules. Limbal stem cells in the form of keratolimbal lenticules were obtained without difficulty. With this technique, thin keratolimbal lenticules of good quality can be obtained efficiently, inexpensively, and without specialized instruments.
Medical subject headings
- Cornea
- Corneal Transplantation
- Epithelium, Corneal
- Limbus Corneae
- Sclera
- Specimen Handling