Chip-based genotyping by mass spectrometry.
basic_science · Level V
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- Record sourced from PubMed, PMID 10468554.
- Also identified by PMC identifier 17834.
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Abstract
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.
Medical subject headings
- DNA
- Genotype
- Polymerase Chain Reaction
- Silicon
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization