Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering.
basic_science · Level V
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- Record sourced from PubMed, PMID 10468571.
- Also identified by PMC identifier 17851.
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Abstract
Time-resolved small-angle x-ray scattering was used to measure the radius of gyration of cytochrome c after initiation of folding by a pH jump. Submillisecond time resolution was obtained with a microfabricated diffusional mixer and synchrotron radiation. The results show that the protein first collapses to compact denatured structures before folding very fast to the native state.
Medical subject headings
- Cytochrome c Group
- Protein Denaturation
- Protein Folding