Store-operated Ca2+ entry: evidence for a secretion-like coupling model.
basic_science · Level V
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- Record sourced from PubMed, PMID 10481913.
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Abstract
The elusive coupling between endoplasmic reticulum (ER) Ca2+ stores and plasma membrane (PM) "store-operated" Ca2+ entry channels was probed through a novel combination of cytoskeletal modifications. Whereas coupling was unaffected by disassembly of the actin cytoskeleton, in situ redistribution of F-actin into a tight cortical layer subjacent to the PM displaced cortical ER and prevented coupling between ER and PM Ca2+ entry channels, while not affecting inositol 1,4,5-trisphosphate-mediated store release. Importantly, disassembly of the induced cortical actin layer allowed ER to regain access to the PM and reestablish coupling of Ca2+ entry channels to Ca2+ store depletion. Coupling is concluded to be mediated by a physical "secretion-like" mechanism involving close but reversible interactions between the ER and the PM.
Medical subject headings
- Calcium
- Calcium Channels
- Calcium Signaling
- Cell Membrane
- Endoplasmic Reticulum
- Ion Channel Gating