Growth of keloid-producing fibroblasts in commercially available serum-free media.

Abstract

The purpose of this study was to update our in vitro serum-free keloid fibroblast (KF) model by use of commercially available media. Prior evaluations of fibroblast characteristics in vitro, especially that of growth factor measurement, have been confounded by the presence of serum-containing media. KFs were obtained from patients undergoing facial keloid removal. The 4 commercially available serum-free media evaluated were AIM-V (Gibco, Grand Island, NY), Fibroblast Growth Medium (FGM; Clonetics, San Diego, CA), HB GRO (Irvine Scientific, Santa Ana, CA), and UltraCULTURE (BioWhittaker Inc, Walkersville, MD). The main outcome measures were sustained KF growth and viability as compared with serum-based models. The KFs in UltraCULTURE had a higher viability but did not grow as well as in FGM. The KFs in HB GRO and AIM-V demonstrated significantly decreased viability. Because of FGM's satisfactory proliferative support and viability comparable with serum-based medium, it is recommended for the in vitro propagation of keloid-producing fibroblasts.