Sequencing complex polysaccharides.
basic_science · Level V
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- Record sourced from PubMed, PMID 10521350.
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Abstract
Although rapid sequencing of polynucleotides and polypeptides has become commonplace, it has not been possible to rapidly sequence femto- to picomole amounts of tissue-derived complex polysaccharides. Heparin-like glycosaminoglycans (HLGAGs) were readily sequenced by a combination of matrix-assisted laser desorption ionization mass spectrometry and a notation system for representation of polysaccharide sequences. This will enable identification of sequences that are critical to HLGAG biological activities in anticoagulation, cell growth, and differentiation.
Medical subject headings
- Glycosaminoglycans
- Oligosaccharides
- Sequence Analysis