Endothelial cell transplantation into the corpus cavernosum: moving towards cell-based gene therapy.

Abstract

As a first step toward a cell based gene therapy for erectile dysfunction, we transplanted fluorescently labeled autologous microvessel endothelial cells (MVEC) into the rat corpus cavernosum. MVEC were isolated from the epididymal fat pad, labeled with the membrane intercalating dye PKH 26, and injected into the corpus cavernosum. Two to 15 days after transplantation the penises were removed, cryosectioned, and examined under epifluorescent and phase contrast microscopy. In 7 consecutive animals transplanted fluorescent cells were identified in the corpora cavernosa. Bilateral distribution was noted in each animal, and staining with ED1 determined that the fluorescence was not due to engulfment of the MVEC by phagocytic cells. Transplanted endothelial cells adhere and persist in the corporal sinusoids and provide a rationale for cell-based gene therapy in the penis.