Improved techniques for kidney transplantation in the monkey.

Abstract

Improved microsurgical techniques for kidney transplantation in the monkey are described. The left graft kidney is transplanted to the lower part of abdomen with end-to-side anastomoses of renal artery to aorta without a patch of aorta, renal vein to inferior vena cava, and end-to-end anastomosis of donor and recipient ureter with 8-0 nylon sutures in a bilateral nephrectomized recipient monkey. We recently performed 60 kidney transplantations in Vervet monkeys. None died of surgery or surgical complications. This reproducible model provides a useful tool to test new immunosuppressants and to investigate the mechanism of drug-induced tolerance and xenotransplantation in primates as a support to clinical trial.