Establishment of a parabiotic rat model by anastomosis of common carotid artery.

Abstract

A cross circulation model was established by replacing mutual blood between two rats by anastomoses of each common carotid artery. In this model, parabiotic rats were shown to replace their mutual blood completely without any artificial materials or instruments, such as silicon tubes or pumps. The rate of blood exchange was measured by intravenous injection of Evans blue dye without the use of the radioactive materials that were so far commonly used. The partial alteration of blood circulation through microvascular surgery may reveal the mechanisms of organ tropism of bacteria, protozoa, and parasites. Microvascular surgery is available for reconstruction and analysis of organ functions as well as for clinical use in organplasty.