True optical resolution beyond the Rayleigh limit achieved by standing wave illumination.
basic_science · Level V
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- Record sourced from PubMed, PMID 10840057.
- Also identified by PMC identifier 16528.
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Abstract
During the last decade, various efforts have been undertaken to enhance the resolution of optical microscopes, mostly because of their importance in biological sciences. Herein, we describe a method to increase the resolution of fluorescence microscopy by illuminating the specimen with a mesh-like interference pattern of a laser source and electronic postprocessing of the images. We achieve 100-nm optical resolution, an improvement by a factor of more than 2 compared with standard fluorescence microscopy and of 1.5 compared with confocal scanning.
Medical subject headings
- Microscopy, Fluorescence