MR imaging of nitrosyl-iron complexes: experimental study in rats.

Abstract

To assess the influence of several nitrosyl-iron complexes on proton nuclear spin relaxation rates to establish a magnetic resonance (MR) imaging technique for nitric oxide. The influence of aqueous phantom solutions of nitrosyl-iron complexes on proton relaxation rates was analyzed for signal enhancement at conventional 1.5-T MR imaging. To induce formation of nitrosyl-iron complexes in a biologic tissue, isolated rat liver was perfused with a saline solution of the NO donor sodium nitroprusside (SNP), and the MR signal intensity was examined thereafter. All investigated nitrosyl-iron complexes shortened the longitudinal, or T1, and transverse, or T2, relaxation times in a concentration-dependent fashion. Relaxivities were highest with a dinitrosyl-iron complex bound to albumin and with a water-soluble mononitrosyl-iron dithiocarbamate complex. The contrast properties of 240 micromol/L of a paramagnetic nitrosyl-iron complex were sufficient to substantially enhance the signal intensity of SNP-perfused rat livers at hydrogen 1 MR imaging. Nitrosyl-iron complexes exhibit a contrast effect at MR imaging that can be exploited for NO imaging in living animals and patients with conventional (1)H MR imaging techniques.