Myosin-V stepping kinetics: a molecular model for processivity.
basic_science · Level V
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- Record sourced from PubMed, PMID 10944217.
- Also identified by PMC identifier 16890.
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Abstract
Myosin-V is a molecular motor that moves processively along its actin track. We have used a feedback-enhanced optical trap to examine the stepping kinetics of this movement. By analyzing the distribution of time periods separating discrete approximately 36-nm mechanical steps, we characterize the number and duration of rate-limiting biochemical transitions preceding each such step. These data show that myosin-V is a tightly coupled motor whose cycle time is limited by ADP release. On the basis of these results, we propose a model for myosin-V processivity.
Medical subject headings
- Calmodulin-Binding Proteins
- Models, Biological
- Molecular Motor Proteins
- Movement
- Myosin Type V
- Nerve Tissue Proteins