Dynamic regulation of the tryptophan operon: a modeling study and comparison with experimental data.
basic_science · Level V
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- Record sourced from PubMed, PMID 11171956.
- Also identified by PMC identifier 29262.
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Abstract
A mathematical model for regulation of the tryptophan operon is presented. This model takes into account repression, feedback enzyme inhibition, and transcriptional attenuation. Special attention is given to model parameter estimation based on experimental data. The model's system of delay differential equations is numerically solved, and the results are compared with experimental data on the temporal evolution of enzyme activity in cultures of Escherichia coli after a nutritional shift (minimal + tryptophan medium to minimal medium). Good agreement is obtained between the numeric simulations and the experimental results for wild-type E. coli, as well as for two different mutant strains.
Medical subject headings
- Bacterial Proteins
- Computer Simulation
- Gene Expression Regulation, Bacterial
- Models, Genetic
- Operon
- Repressor Proteins
- Tryptophan