Stereotactic core biopsy breast and blood cell by-products: a source of material for molecular genetics research--initial experience.

Stomper, P C; Stewart, C C; Stoler, D L · Radiology · 2001

basic_science · Level V

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Abstract

Stereotactic core biopsy washings and blood drop samples, routinely discarded by-products, provide satisfactory fresh cellular material for flow cytometry and molecular genetics microsatellite polymerase chain reaction (PCR) analysis for detection of loss of DNA alleles (loss of heterozygosity). Cytokeratin-positive (epithelial) cells from the core biopsy washings were sorted by means of flow cytometry prior to PCR analysis. DNA allele loss was detected in benign breast epithelial cells in three (20%) of 15 patients.

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