Intralymphatic immunization enhances DNA vaccination.
basic_science · Level V
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- Record sourced from PubMed, PMID 11248073.
- Also identified by PMC identifier 30648.
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Abstract
Although DNA vaccines have been shown to elicit potent immune responses in animal models, initial clinical trials in humans have been disappointing, highlighting a need to optimize their immunogenicity. Naked DNA vaccines are usually administered either i.m. or intradermally. The current study shows that immunization with naked DNA by direct injection into a peripheral lymph node enhances immunogenicity by 100- to 1,000-fold, inducing strong and biologically relevant CD8(+) cytotoxic T lymphocyte responses. Because injection directly into a lymph node is a rapid and easy procedure in humans, these results have important clinical implications for DNA vaccination.
Medical subject headings
- Antigens, Viral
- DNA, Viral
- Glycoproteins
- Lymphocytic choriomeningitis virus
- Peptide Fragments
- Vaccines, DNA
- Viral Proteins
- Viral Vaccines