Single-molecule observation of protein-protein interactions in the chaperonin system.
basic_science · Level V
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Abstract
We have analyzed the dynamics of the chaperonin (GroEL)-cochaperonin (GroES) interaction at the single-molecule level. In the presence of ATP and non-native protein, binding of GroES to the immobilized GroEL occurred at a rate that is consistent with bulk kinetics measurements. However, the release of GroES from GroEL occurred after a lag period ( approximately 3 s) that was not recognized in earlier bulk-phase studies. This observation suggests a new kinetic intermediate in the GroEL-GroES reaction pathway.
Medical subject headings
- Chaperonin 10
- Chaperonins
- Microscopy, Fluorescence