Fluorescence in situ hybridization analysis of peripheral blood cells in Pearson marrow-pancreas syndrome.

Yanagihara, I; Inui, K; Yanagihara, K; Park, Y D; Tanaka, J; Ozono, K; Okada, S; Kurahashi, H · J Pediatr · 2001

basic_science · Level V

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Abstract

We used a dual-color fluorescence in situ hybridization technique to estimate deleted mitochondrial DNA at a single-cell level and determine any correlation with the disease progression in lymphocytes from patients with Pearson marrow-pancreas syndrome. The method demonstrated a shift in heteroplasmy, paralleling the hematologic improvement.

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