CD5 expression by B lymphocytes and its regulation upon Epstein-Barr virus transformation.

Kaplan, D; Smith, D; Meyerson, H; Pecora, N; Lewandowska, K · Proc Natl Acad Sci U S A · 2001

basic_science · Level V

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Abstract

Dim expression of CD5 on human B lymphocytes has been used to delineate B1 and B2 subsets. Nevertheless, others have suggested that the molecule is an activation marker and does not predicate a subset distinction. We have used enzymatic amplification staining, a technology that enhances the resolution of flow cytometric analysis of cell surface molecules by as much as 100-fold, to determine that essentially all human B cells express CD5. Furthermore, we show that this expression is regulated during Epstein-Barr virus transformation.

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