Avoiding cross hybridization by choosing nonredundant targets on cDNA arrays.
basic_science · Level V
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Abstract
PROBEWIZ designs PCR primers for amplifying probes for cDNA arrays. The probes are designed to have minimal homology to other expressed sequences from a given organism. The primer selection is based on user-defined penalties for homology, primer quality, and proximity to the 3' end.
Medical subject headings
- Oligonucleotide Array Sequence Analysis
- Software