Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis.
basic_science · Level V
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- Record sourced from PubMed, PMID 11867724.
- Also identified by PMC identifier 122409.
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Abstract
We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the approximately 800 inner-membrane proteins thought to exist in E. coli.
Medical subject headings
- Bacterial Proteins
- Cyclin-Dependent Kinases
- Escherichia coli
- Membrane Proteins