A bioinformatic strategy to rapidly characterize cDNA libraries.
other · Level V
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Abstract
Complementary DNA libraries can define the genetic constituents of specific cells and/or tissues. Their sequencing will illuminate the transcriptome but it is a monumental task requiring considerable resources. We have employed a computational search in conjunction with a microarray-based strategy to alleviate the impediments of deriving a consensus of records that describe testis gene expression. This strategy identified 5681 unique testes-expressed genes of which 3265 were previously portrayed in the UniGene database. Interestingly, a total of 2416 novel testes-expressed genes were identified. This clearly demonstrates that microarrays can be used to rapidly discover a large number of new transcripts.
Medical subject headings
- Databases, Nucleic Acid
- Gene Expression
- Gene Expression Profiling
- Gene Library
- Oligonucleotide Array Sequence Analysis