The mechanism of type IA topoisomerases.
basic_science · Level V
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- Record sourced from PubMed, PMID 12167668.
- Also identified by PMC identifier 129409.
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Abstract
The topology of cellular DNA is carefully controlled by enzymes called topoisomerases. By using single-molecule techniques, we monitored the activity of two type IA topoisomerases in real time under conditions in which single relaxation events were detected. The strict one-at-a-time removal of supercoils we observed establishes that these enzymes use an enzyme-bridged strand-passage mechanism that is well suited to their physiological roles and demonstrates a mechanistic unity with type II topoisomerases.
Medical subject headings
- DNA Topoisomerases, Type I