ShiftDetector: detection of shift mutations.
basic_science · Level V
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- Record sourced from PubMed, PMID 12176839.
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Abstract
Sequencing of a bi-allelic PCR product, which contains an allele with a deletion/insertion mutation results in a superimposed tracefile following the site of this shift mutation. A trace file of this type hampers the use of current computer programs for base calling. ShiftDetector analyses a sequencing trace file in order to discover if it is a superimposed sequence of two molecules that differ in a shift mutation of 1 to 25 bases. The program calculates a probability score for the existence of such a shift and reconstructs the sequence of the original molecule. ShiftDetector is available from http://cowry.agri.huji.ac.il
Medical subject headings
- DNA Mutational Analysis
- Genetic Carrier Screening
- Mutagenesis, Insertional
- Polymerase Chain Reaction
- Software