Cytochrome c folding pathway: kinetic native-state hydrogen exchange.
basic_science · Level V
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- Record sourced from PubMed, PMID 12196629.
- Also identified by PMC identifier 129417.
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Abstract
Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each. Results obtained define a cytochrome c intermediate seen only poorly before and place it early on the major unfolding pathway. Four distinct unfolding steps are found to be kinetically ordered in the same pathway sequence inferred before.
Medical subject headings
- Cytochrome c Group