Molecular analysis of microscopic ezrin dynamics by two-photon FRAP.
basic_science · Level V
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- Record sourced from PubMed, PMID 12271120.
- Also identified by PMC identifier 130542.
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Abstract
Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photobleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.
Medical subject headings
- Phosphoproteins
- Spectrometry, Fluorescence