Regulation of elongating RNA polymerase II by forkhead transcription factors in yeast.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 12702877.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The elongation phase of transcription by RNA polymerase II (RNAPII) is highly regulated and tightly linked to pre-messenger RNA (pre-mRNA) processing. Recent studies have implicated an early elongation checkpoint that facilitates the link to pre-mRNA processing. Here we show that the yeast forkhead transcription factors, Fkh1p and Fkh2p, associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination. These events are coordinated with serine-5 and -2 phosphorylation of the heptad repeat of the carboxy-terminal domain (CTD) of RNAPII. Our results suggest that, in addition to their documented promoter function, Fkh1p and Fkh2p coordinate early transcription elongation and pre-mRNA processing. This may reflect a general feature of gene regulation in eukaryotes.
Medical subject headings
- Cell Cycle Proteins
- Genes, Fungal
- RNA Polymerase II
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors
- Transcription, Genetic
- Uracil