Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription.

Bushnell, David A; Kornberg, Roger D · Proc Natl Acad Sci U S A · 2003

basic_science · Level V

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Abstract

The x-ray structure of complete RNA polymerase II from Saccharomyces cerevisiae has been determined, including a heterodimer of subunits Rpb4 and Rpb7 not present in previous "core" polymerase II structures. The heterodimer maintains the polymerase in the conformation of a transcribing complex, may bind RNA as it emerges from the enzyme, and is in a position to interact with general transcription factors and the Mediator of transcriptional regulation.

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