Production of knockout rats using ENU mutagenesis and a yeast-based screening assay.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 12754522.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes.
Medical subject headings
- Ethylnitrosourea
- Gene Expression Regulation
- Gene Targeting
- Mutagenesis, Site-Directed
- Rats, Mutant Strains