Germ-line transgenesis of the Tc1/mariner superfamily transposon Minos in Ciona intestinalis.
basic_science · Level V
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- Record sourced from PubMed, PMID 12788975.
- Also identified by PMC identifier 164655.
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Abstract
The tadpole larva of the basal chordate Ciona intestinalis has the most simplified, basic body-plan of chordates. Because it has a compact genome with a complete draft sequence, a large quantity of EST/cDNA information, and a short generation time, Ciona is a suitable model for future genetics. We establish here a transgenic technique in Ciona that uses the Tc1/mariner superfamily transposon Minos. Minos was integrated efficiently into the genome of germ cells and transmitted stably to subsequent generations. In addition, an enhancer-trap line was obtained. This is a demonstration of efficient, Minos-mediated transgenesis in marine invertebrates.
Medical subject headings
- Animals, Genetically Modified
- Ciona intestinalis
- DNA Transposable Elements
- DNA-Binding Proteins
- Genetic Techniques
- Transposases