Bonded interface between a self-curing resin and dentin primed with a metalloprotein.

Abstract

The purpose of this study was to investigate adhesive bonding between a self-curing luting agent and dentin conditioned with a metalloprotein in terms of resin-dentin hybridization and interfacial polymerization. Of the six experimental primers containing bovine heart cytochrome c (BHCC), three contained 2-hydroxyethylmethacrylate (HEMA) and the remaining three did not. The self-curing luting agent used consisted of methyl methacrylate (MMA) and tri-n-butylborane (TBB) with or without 4-methacryloyloxyethyl trimellitate anhydride (4-META). Bovine dentin surfaces were etched with 10wt% phosphoric acid, primed, and then bonded with stainless steel rods. After 24 h of water storage, the optimum bond strength was obtained with the 4-META/MMA-TBB luting agent and the aqueous primer contained 0.1 micromol/g BHCC and 35wt% HEMA. Microscopic observations showed continuity among the luting agent, the hybridized dentin, and the dentin substrate. A model experiment suggested that BHCC accelerates the polymerization of the 4-META/MMA/HEMA mixture from the hybridized dentin.