Protein-DNA interaction mapping using genomic tiling path microarrays in Drosophila.
basic_science · Level V
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- Record sourced from PubMed, PMID 12876199.
- Also identified by PMC identifier 170935.
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Abstract
We demonstrate the use of a chromosomal walk (or "tiling path") printed as DNA microarrays for mapping protein-DNA interactions across large regions of contiguous genomic DNA in Drosophila melanogaster. Microarrays were constructed with genomic DNA fragments 430-920 bp in length, covering 2.9 million base pairs of the Adh-cactus region of chromosome 2 and 85,000 base pairs of the 82F region of chromosome 3. We performed DNA localization mapping for the heterochromatin protein HP1 and for the sequence-specific GAGA transcription factor, producing a comprehensive, high-resolution map of in vivo protein-DNA interactions throughout these regions of the Drosophila genome.
Medical subject headings
- DNA
- DNA-Binding Proteins
- Drosophila
- Oligonucleotide Array Sequence Analysis