MPO activity and generation of active O2 species in leukocytes from poorly controlled diabetic patients.

Abstract

This study was undertaken to determine which part of ROI generation is reduced in the neutrophils from patients with NIDDM. Superoxide anion (O2-) production, LDCL activity in response to opsonized zymosan, and MPO activity were measured in leukocytes of poorly controlled NIDDM patients (FBG greater than 8.89 mM, HbA1 greater than 10%). In diabetic subjects, both O2- production and LDCL activity assessed with initial slope gradient and peak value were significantly reduced. MPO activity was also decreased in diabetic subjects, and there was a significant correlation between HbA1 levels and MPO activity of diabetic subjects. This study demonstrated that every step in leukocyte ROI generation should be reduced in the leukocytes from poorly controlled diabetic patients.