In situ demonstration of Epstein-Barr virus in intravenous drug abusers with generalized lymphadenopathy.

Mampaso, F; Bellas, C; Molina, A; Quereda, C; Bricio, T; Buzon, L · Postgrad Med J · 1992

case_series · Level IV

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Abstract

We have studied by the in situ hybridization method the presence of Epstein-Barr virus (EBV) DNA genome in lymph node tissues from 11 patients with persistent generalized lymphadenopathy. Using a biotinylated EBV DNA probe, we demonstrated EBV nucleic acid in scattered germinal centre cells in eight of the 11 cases. Our results suggest that EBV is not a determinant factor in the pathogenesis of this lymphadenopathy, but support its possible implication in B cell malignant transformation in cases of AIDS-associated lymphoma.

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