Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 1347950.
- Also identified by PMC identifier 48677.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.
Medical subject headings
- Chromosome Mapping
- Chromosomes, Fungal
- DNA
- Gene Library
- Saccharomyces cerevisiae