Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.
basic_science · Level V
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- Record sourced from PubMed, PMID 1379716.
- Also identified by PMC identifier 49573.
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Abstract
Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.
Medical subject headings
- Amino Acid Oxidoreductases
- Brain
- Isoenzymes
- Macrophages
- Neurons