Immobilization of alpha-amylase into photographic gelatin by chemical cross-linking.
basic_science · Level V
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- Record sourced from PubMed, PMID 1420716.
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Abstract
alpha-Amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.
Medical subject headings
- Cross-Linking Reagents
- Enzymes, Immobilized
- Gelatin
- alpha-Amylases