Oxyhemoglobin dissociation during hypothermic blood cardioplegia arrest.

Abstract

Coronary sinus effluent contains desaturated blood during the first few seconds of hypothermic cardioplegia infusion in humans. This occurs despite the high affinity of hemoglobin for oxygen at a low temperature and alkaline pH. The present study quantitates oxyhemoglobin dissociation during hypothermic cardioplegic arrest. Three infusions of a 4 degrees C alkalotic blood cardioplegia solution were given into the cross-clamped aortic root during 1 hour of cardioplegic arrest. Paired aortic root and coronary sinus blood samples were obtained before and shortly after initiating cardiopulmonary bypass and at t = 5 seconds and 30 seconds during each cardioplegia infusion. Throughout the study, the hemoglobin saturation in the aortic root samples was 100%. The mean coronary sinus hemoglobin saturation at t = 5 seconds during hypothermic cardioplegia infusion ranged from 63.0% to 66.5% (p < 0.05 coronary sinus compared with aortic root samples). The coronary sinus hemoglobin saturation approximated the aortic root hemoglobin saturation at t = 30 seconds during hypothermic cardioplegia infusion. The mean PO2 of the aortic root samples ranged from 214 to 307 mm Hg during hypothermic cardioplegia infusion. The mean PO2 of the t = 5 seconds coronary sinus samples ranged from 31 to 39 mm Hg, whereas the mean PO2 of the t = 30 seconds coronary sinus samples ranged from 85 to 119 mm Hg during cardioplegia infusion (p < 0.05 coronary sinus compared with aortic root samples). Oxygen dissociates from hemoglobin contained in a hypothermic, alkalotic blood cardioplegia solution during the nonperfused phase of cardioplegic arrest. However, the only oxygen delivered to the myocardium during the infusion of a hypothermic alkalotic blood cardioplegia solution is oxygen physically dissolved in the solution.