Somatic recombination of heavy chain variable region transgenes with the endogenous immunoglobulin heavy chain locus in mice.
basic_science · Level V
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- Record sourced from PubMed, PMID 1438216.
- Also identified by PMC identifier 50330.
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Abstract
Transgenic lines of mice were derived by using plasmid constructs containing DNA encoding an antibody heavy chain variable-diversity-joining region (VH-D-JH) and various amounts of 5' and 3' flanking DNA but lacking any repetitive isotype switch (S) or constant (C) region DNA. Unexpectedly, many of the antibody VH regions expressed by B-cell hybridomas generated from immunized transgenic mice were found to be of transgenic origin. Further analyses showed that somatic events had generated hybrid genomic loci in the mice containing the transgenic VH-D-JH gene and plasmid sequences 5' of endogenous heavy chain C region genes. Thus, VH-D-JH transgenes lacking S and C region DNA can recombine with endogenous Igh DNA, leading to the expression of transgene-encoded antibody.
Medical subject headings
- Genes, Immunoglobulin
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region