Oral administration of beta-lactamase preserves colonization resistance of piperacillin-treated mice.

Stiefel, Usha; Pultz, Nicole J; Harmoinen, Jaana; Koski, Pertti; Lindevall, Kai; Helfand, Marion S; Donskey, Curtis J · J Infect Dis · 2003

basic_science · Level V

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Abstract

We hypothesized that orally administered, recombinant class A beta-lactamase would inactivate the portion of parenteral piperacillin excreted into the intestinal tract, preserving colonization resistance of mice against nosocomial pathogens. Subcutaneous piperacillin or piperacillin plus oral beta-lactamase were administered 24 and 12 h before orogastric inoculation of piperacillin-resistant pathogens. Oral administration of beta-lactamase reduced piperacillin-associated alteration of the indigenous microflora and prevented overgrowth of pathogens.

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