Thermodynamic treatment of oligonucleotide duplex-simplex equilibria.
basic_science · Level V
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- Record sourced from PubMed, PMID 14657395.
- Also identified by PMC identifier 299820.
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Abstract
Thermodynamic formulations have been devised to obtain Delta G degrees values directly from spectroscopic data at a fixed common temperature in nucleic acid duplex-simplex melting curves. In addition, the dependence of melting on salt concentration has been expressed in terms of a stepwise stoichiometric representation, which leads to a specific equation for the partition of the added sodium ions between the different oligonucleotide forms.
Medical subject headings
- Nucleic Acid Conformation
- Nucleic Acid Denaturation
- Oligonucleotides