Hepatitis C virus-derived lipopeptides differentially induce epitope-specific immune responses in vitro.
basic_science · Level V
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Abstract
We studied the induction of T cell responses against hepatitis C virus (HCV)-related cytotoxic T cell (CTL) epitopes in lymphocytes from human leukocyte antigen (HLA)-A2-positive, HCV-naive individuals. Peptide and lipopeptides corresponding to the epitope at amino acids (aa) 1073-1081 induced equivalent numbers of tetramer-positive CD8+ T cells and detectable interferon-gamma and granzyme B spot-forming cells (sfcs). Epitope aa 35-44 induction of CTLs was not achieved, despite detectable tetramer-positive CD8+ T cells. IFN-gamma sfcs could only be induced with lipopeptide aa 20-44 but not by conventional antigens, which indicates that lipopeptides may alter T cell functions, depending on the epitope.
Medical subject headings
- Epitopes, T-Lymphocyte
- Hepacivirus
- Lipoproteins
- Peptide Fragments
- T-Lymphocytes, Cytotoxic
- Viral Core Proteins
- Viral Nonstructural Proteins