ROSO: optimizing oligonucleotide probes for microarrays.

Reymond, Nancie; Charles, Hubert; Duret, Laurent; Calevro, Federica; Beslon, Guillaume; Fayard, Jean-Michel · Bioinformatics · 2004

other · Level V

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Abstract

ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set. The program is available on the internet. Sources are freely available, for non-profit use, on request to the authors. http://pbil.univ-lyon1.fr/roso

Medical subject headings