Sequence-selective DNA detection using multiple laminar streams: a novel microfluidic analysis method.
basic_science · Level V
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- Record sourced from PubMed, PMID 15007428.
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Abstract
On-site detection methods for DNA have been demanded in the pathophysiology field. Such analysis requires a simple and accurate method, rather than high-throughput. This report describes a novel microfluidic analysis method and its application for simple sequence-selective DNA detection. The method uses a microchannel device with a serpentine structure. Sequence-specific binding of probe DNA can be detected at one side of the microchannel. This method is capable of sequence-specific detection of DNA with high accuracy. Single base mutations can also be analyzed. Combination of laminar stream and laminar secondary flow in the microchannel enable specific detection of probe-bound DNA.
Medical subject headings
- Genetic Techniques
- Microfluidics
- Sequence Analysis, DNA