Sub-second isoelectric focusing in free flow using a microfluidic device.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 15007450.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Using a microfabricated chip with a bed volume of 0.2 microL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.
Medical subject headings
- Isoelectric Focusing
- Microcomputers
- Nanotechnology