Microchip countercurrent electroseparation.

Ista, Linnea K; Lopez, Gabriel P; Ivory, Cornelius F; Ortiz, Moises J; Schifani, Thomas A; Schwappach, Christopher D; Sibbett, Scott S · Lab Chip · 2003

basic_science · Level V

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Abstract

We report a microchip-based method for separating charged molecules according to electrophoretic mobility. The method is based on opposed electroosmotic, electrophoretic and convective forces. Similar to isoelectric focusing, solute can be accumulated into stationary zones, but without use of ampholytes. The method of "microchip countercurrent electroseparation" described here has potential application in the design of microfluidic separation chips.