Accelerated induction of bladder cancer in patched heterozygous mutant mice.

Hamed, Sahar; LaRue, Hélène; Hovington, Hélène; Girard, Johanne; Jeannotte, Lucie; Latulippe, Eva; Fradet, Yves · Cancer Res · 2004

basic_science · Level V

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Abstract

The PATCHED (PTC) gene is recognized as a tumor suppressor in basal cell carcinoma. Mapping of a minimal region of deletion at 9q22.3 and observation of a decreased PTC expression in superficial papillary bladder tumors led us to hypothesize that it could also be involved in this cancer. To further investigate this hypothesis, we submitted Ptc(+/-) heterozygous mutant mice and their wild-type littermates to chemical carcinogenesis by adding N-butyl-N-(4-hydroxybutyl) nitrosamine to their drinking water. Preneoplastic and neoplastic changes were observed significantly earlier in the Ptc(+/-) than in the wild-type mice. Our data support the hypothesis of Ptc acting as a tumor suppressor gene in bladder cancer.

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