PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR.
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- Record sourced from PubMed, PMID 15073005.
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Abstract
PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design. Open-source and freely available from http://perlprimer.sourceforge.net.
Medical subject headings
- Algorithms
- Chromosome Mapping
- DNA Primers
- Polymerase Chain Reaction
- Sequence Analysis, DNA
- Software
- User-Computer Interface